Determining optimum in vitro mineral nutrition for diverse pear germplasm using response surface methodology
Complex chemical interactions in media and variation in genotype response make it very difficult to optimize mineral nutrition of in vitro plants. Plant growth response of diverse pear genotypes on standard tissue culture medium varies widely. A group of studies were designed to determine the mineral nutrient requirements for micropropagation of pear shoots from this diverse collection. Initially, five mineral nutrient factors were created from MS salts NDASH NH4NO3, KNO3, mesos components (Ca-Mg-Cl-Mn-SO4-PO4), metals (Zn-Mn-Cu-Co-Mo-B-I), and Fe-EDTA. Each factor was varied over a range of concentrations. Treatment combinations were selected using response surface methodology (RSM). Five pear genotypes in three species (Pyrus communis, P. dimorphophylla and P. ussuriensis) were grown on each treatment combination. Measurements included shoot quality, severity of leaf spotting/necrosis, leaf size, shoot number, and shoot length, and each response was analyzed by ANOVA with results modeled using RSM. Models for each genotype identified the factors with the largest growth responses. Additional experiments, testing mesos and nitrogen components, determined the optimal concentrations of these factors for a wider range of pear species and cultivars. Mineral nutrient formulations were identified that resulted in significantly better growth for all the genotypes tested. This approach was very successful for defining new media for shoot proliferation of diverse pear germplasm.
Reed, B.M., DeNoma, J., Wada, S. and Niedz, R. 2016. Determining optimum in vitro mineral nutrition for diverse pear germplasm using response surface methodology. Acta Hort. (ISHS) 1113:79-84
micropropagation, medium optimization, modelling, Pyrus