In vitro propagation of Lippia citriodora

M. Papafotiou, N. Tsalouchos, A. Papagianni, K.F. Bertsouklis
In vitro cultures were established from node explants excised from adult plants and cultured on MS medium with 30 g L-1 sucrose, supplemented with N6-benzyladenine (BA) and naphthalene acetic acid (NAA) at various concentrations. At the establishment stage the highest response for shoot production was recorded on MS medium supplemented with 1.0 mg L-1 BA and 0.01 mg L-1 NAA. At two following subcultures higher BA concentration, as well as zeatin (ZEA), were tested as for their effect on shoot multiplication. The increase of BA concentration from 1 to 2 mg L-1 did not affect multiplication; neither did the use of 2 mg L-1 ZEA instead of BA in terms of multiplication index. Similarly to the establishment stage, the combination 1 mg L-1 BA/ 0.01 mg L-1 NAA induced good response, giving 2-3 shoots explant-1 with 3 nodes shoot-1, that allowed successful continuation of the in vitro culture and quite good shoot proliferation. Microshoots rooted at 67% on MS medium with 1 mg L-1 IBA. Ex vitro acclimatization was successful (83%) on a mixture of peat:perlite (1:1, v/v).
Papafotiou, M., Tsalouchos, N., Papagianni, A. and Bertsouklis, K.F. 2017. In vitro propagation of Lippia citriodora. Acta Hort. (ISHS) 1155:327-330
cytokinin, ex vitro acclimatization, in vitro rooting, lemon verbena, multiplication

Acta Horticulturae