Temporary immersion bioreactors for large scale Globba micropropagation
Temporary immersion bioreactor (TIB) system is applicable for a large-scale and rapid micropropagation at industrial level. This research aimed at establish a culture system for Globba propagation comparing the use of 6-L polyethylene terephthalate (PET) container sealed with lid made of high-density polyethylene (HDPE) versus 20-L polycarbonate (PC) and 700-mL glass containers. The benefits of PET container are its low weight, flexibility, relative tolerance to external handlings and the extremely lower cost (5 USD) compared with PC type (300 USD). The first step of this study focused on a sterilization method of the PET containers using hydrogen peroxide (H2O2) at 0.1, 0.3, 1 and 3% coupled or not with U.V.-C exposure. The most suitable sterilization treatment was performed by rinsing PET container with 0.3% H2O2 for 1 min. A second step was addressed to evaluate the propagation efficiency of Globba in vitro shoots using TIB culture with PET or PC containers and glass containers with solid medium used as a control. The basal medium to assess the in vitro growth was MS medium supplemented with 0.025 mg L-1 Thidiazuron (TDZ). With TIB cultures, shoots were immersed in 4 L medium over 6 times day-1 for a duration of 5 min each time. After a 4-week-period culture, results showed that with TIB-PET system 1,100 plants were obtained. At the same time, the TIB-PC system and the solid medium allowed to have 913 and 530 plants, respectively. Plants propagated in TIB system performed better compared to shoots grown in solid medium. The culture area is much more productive with TIB-PC (4.72 times) and TIB-PET (14.94 times) than glass container.
Topoonyanont, N., Pumisutapon, P., Klayraung, S. and Poonnoy, P. 2017. Temporary immersion bioreactors for large scale Globba micropropagation. Acta Hort. (ISHS) 1155:51-58
hydrogen peroxide, disinfection, plant propagation, TIB system, plastic containers, culturing area