Micropropagation of Spathoglottis eburnea Gagnep, a Thai orchid species, through shoot tips
Spathoglottis eburnea Gagnep is a Thai terrestrial orchid species grown in the north and northeast. It has a small population in the wild and can be endangered in the near future. To develop a protocol for Spathoglottis eburnea micropropagation, shoot tips were proliferated in half strength Murashige and Skoog (½MS) liquid media supplemented with the combination of α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) (0, 0.5, 1 or 2 mg L-1) and placed on a shaker at 110 rpm. After 3 months of culture, the highest shoot formation of 30.8 shoots explant-1 was obtained on ½MS medium supplemented with 1 mg L-1 NAA and 2 mg L-1 BAP. Whereas, the combination of 2 mg L-1 NAA and 0.5 mg L-1 BAP gave the highest root formation of about 11.1 roots explant-1. Moreover, the regenerated plants were analyzed by using flow cytometry and the results showed the same ploidy level.
Pornchuti, W., Thammasiri, K., Chuenboonngarm, N. and Panvisavas, N. 2017. Micropropagation of Spathoglottis eburnea Gagnep, a Thai orchid species, through shoot tips. Acta Hort. (ISHS) 1167:87-94
propagation, NAA, BAP, conservation, flow cytometry