The alternative use of foliar fertilizers as a modified medium for in vitro culture of Dendrobium Fleischeri

K. Obsuwan, S. Tharapan, C. Thepsithar
Previous experiment indicated that Hyponex fertilizer was suitable for in vitro culture of Dendrobium hybrids. However, Hyponex is not available in Thailand. Thus, in this experiment we aimed to compare 5 different commercial foliar fertilizers with Hyponex as culture media. Dendrobium Fleischeri shoots were cultured on semi-solid medium containing each of 6 different foliar fertilizers; Hyponex (6.5-6-19), MEGA-FER® (10-20-30), Nutra-Phos Super-K® (7-13-34), Tutur® (6-32-32), Biomer® (7-24-34) or Polean Golden Orchid® (10-52-17) at the concentration of 0.35% (w/v) supplemented with 2.0% (w/v) sucrose, 0.2% (w/v) phytagel and 0.5 g L-1 activated charcoal and cultured for 3 months. The results showed no significant differences in survival rate and number of shoots in all treatments, but no survival was found from shoots cultured on Polean Golden Orchid® (10-52-17). The maximum fresh and dry weight, as well as number of roots were obtained from shoots cultured on Nutra-Phos Super-K® (7-13-34) and Tutur® (6-32-32); however, Nutra-Phos Super-K® (7-13-34) provided the best root length. The study suggested that Nutra-Phos Super-K® (7-13-34) can be used as a substitute for Hyponex (6.5-6-19) in culture media of Dendrobium hybrid.
Obsuwan, K., Tharapan, S. and Thepsithar, C. 2017. The alternative use of foliar fertilizers as a modified medium for in vitro culture of Dendrobium Fleischeri. Acta Hort. (ISHS) 1167:115-118
http://www.actahort.org/books/1167/1167_17.htm
Hyponex, Dendrobium hybrid, tissue culture, fresh weight
English

Acta Horticulturae