Role of plant growth regulators on fruit set and embryo culture of interspecific Phalaenopsis

K. Rungruchkanont, T. Promchot
Phalaenopsis buyssoniana is a species in the Phalaenopsis genus that has high chromosome numbers (2n=4x=76); however, the problem occurred when crossed with P. 'Wedding Promenade'. The cross has low fruit set. In order to overcome this restriction, plant growth regulators were used in this study for 2 phases; 1) applying in vivo after cross pollination to promote fruit set; and 2) adding into the culture medium to enhance efficiency of embryo rescue. Auxin treatments (NAA, IAA or 2,4-D) were applied after cross pollination to promote fruit set of P. buyssoniana and P. 'Wedding Promenade'. P. 'Wedding Promenade', a female parent, produced the highest fruit set, increased capsule size and rescued embryos when 2,4-D was applied. The study of capsule age and culture medium were conducted in order to enhance efficiency of embryo rescue. The capsule ages were 1, 1.5, 2 and 2.5 months. The culture media were based on Vacin and Went medium (control) and the other 4 treatments were 1) 100 mg L-1 2,4-D; 2) 50 mg L-1 Dicamba; 3) 1 mg L-1 Kinetin plus 0.1 mg L-1 NAA; and 4) 2 g L-1 Peptone. The two-month-old capsules, cultured in Vacin and Went and supplemented with 1 mg L-1 Kinetin plus 0.1 mg L-1 of NAA, were found highly effective, producing 100% capsule development with 82.6 developed embryos per capsule.
Rungruchkanont, K. and Promchot, T. 2017. Role of plant growth regulators on fruit set and embryo culture of interspecific Phalaenopsis. Acta Hort. (ISHS) 1167:119-126
http://www.actahort.org/books/1167/1167_18.htm
auxin, embryo rescue, fruit set, hybrid, Phalaenopsis orchid
English

Acta Horticulturae