Effect of two bio polysaccharides on organogenesis of PLBs in Dendrobium kingianum cultured in vitro
Growth and development of PLBs cultured in vitro are largely dependent on the presence of different plant growth chemicals. In this study, potential effect of two biopolymer: hyaluronic acid (HA9; Shiseido, Japan) and sodium alginate (ULV-13, Kimica, Japan) on organogenesis of protocorm-like bodies (PLBs) in Dendrobium kingianum under white fluorescent lamp was investigated. PLBs of D. kingianum were explanted on modified MS medium supplemented with various concentrations of HA9 and sodium alginate. In case of HA9, the highest number of PLBs (15.2) was recorded at concentration of 0.1 mg L-1. This study shows that very low concentration of sodium alginate (0.01 mg L-1) increased the number of PLBs dramatically and that was 23.6 compared with control (9.6). Fresh weight was also highest at same concentration, while the highest number of shoots explant-1 (3.7) was observed at concentration of 10 mg L-1 sodium alginate. The results indicated that the application of HA9 and sodium alginate and their combination at a low concentration increased average number of PLBs but shoot formation was highest at high concentration compared with control.
Habiba, S.U., Shimasaki, K., Ahasan, M.M. and Uddin, A.F.M.J. 2017. Effect of two bio polysaccharides on organogenesis of PLBs in Dendrobium kingianum cultured in vitro. Acta Hort. (ISHS) 1167:127-132
Dendrobium, protocorm-like bodies (PLBs), hyaluronic acid (HA9), sodium alginate