A cost effective in vitro culture protocol of Dendrobium Fleischeri
Agar is the most commonly used for solidifying media in plant tissue culture. However, several studies indicated that the differences in agar types and concentrations showed significant difference in quality of in vitro plantlets. In these studies, 3 different gelling agents, Phytagel, Agar and CleriGar, were compared on growth and development of Dendrobium Fleischeri seedlings. After culturing for 2 months, the results showed that gelling agent types and concentrations showed no effect on fresh and dry weight, number of roots and shoots and root length of in vitro-grown Dendrobium Fleischeri plantlets. Moreover, conditions of culture media, liquid and semi-solid were compared in order to reduce the cost according to the expensive price of gelling agents. It was found that Dendrobium Fleischeri shoots cultured in liquid medium gave a significantly greater fresh and dry weight, number of roots and shoots and root length than those obtained from semi-solid medium containing those 3 gelling agents. Thus, the results of this study suggest a new possibility of using liquid medium to reduce production cost considerably and will be the promising in vitro culture technique for growth and development of Dendrobium orchid.
Obsuwan, K., Tharapan, S. and Thepsithar, C. 2017. A cost effective in vitro culture protocol of Dendrobium Fleischeri. Acta Hort. (ISHS) 1167:139-142
cost-reducing, hyperhydricity, gelling agents, liquid medium