Initial study on 2n gametes induction of Strelitzia reginae
By using sexual polyploidization method to create new Strelitzia reginae germplasm, young flowers which are about 6-8 cm in length are induced with different concentrations of colchicine (0.05, 0.10 and 0.20%) in the first meiosis. The results showed that the effects of 0.20% colchicine were the best. On the basis of morphological observations, the differences were obvious, such as petal, calyx, stigma, pollen grain germination, and so on. Based on cytological study, abnormal meiosis behavior resulting in the 2n pollen production showed that: (1) abnormal spindles, including parallel spindles and fused spindles were formed at metaphase II and anaphase II; (2) the nuclei at telophase II were arranged in two poles, each pole contained two nuclei, or in three poles, one of which contained two nuclei, the other pole contained one nucleus, respectively; (3) dyads and triads were produced at the tetrad stage. According to the cytological mechanism of 2n pollen formation, the results indicated that the 2n pollen were genetically equivalent to the first division restitution (FDR) gametes and consequently had a potential value for sexual polyploidization breeding of S. reginae.
Zheng, S.X., Liao, X.S., Lin, Q.D., Ling, W.B., Gong, J.H., Long, B., Yang, Y.H., Zhou, J., Wang, L.Y. and Deng, Y.W. 2017. Initial study on 2n gametes induction of Strelitzia reginae. Acta Hort. (ISHS) 1167:157-162
Strelitzia reginae, colchicines, 2n gametes, meiosis