Fungal chitosan for growth and PAL gene expression of Dendrobium Sonia 'Bom 17' in vitro
Orchids are among the largest group of the flowering plants in the world. Dendrobium Sonia LSQUOBom 17RSQUO is one of the most popular orchid hybrids and important for agricultural industries in Thailand. Hence, their high productivity via tissue culture techniques combined with various plant elicitors, such as chitosan is needed. This research focused on the effects on Denbrobium Sonia 'Bom 17' growth rates and PAL gene expression following treatments with fungal chitosan of different molecular weights and concentrations. The results showed that low molecular weight chitosan at 50 and 100 mg L-1 could promote shoot and root length, respectively; while, low molecular weight chitosan at 10 mg L-1 was more effective than high molecular weight chitosan at this concentration for promoting new shoots. However, high molecular weight chitosan was more effective in promoting the appearance of new leaves. Expression of the PAL gene, which is normally expressed when plants are under stressed conditions following fungal infection, was also studied. The results showed that, the PAL gene expression in the shoot of Dendrobium Sonia 'Bom 17' is at its highest 48 h after treatment with 50 mg L-1 of low molecular weight chitosan.
Nakkuntod, M., Chinanukulwong, S., Kongbangkerd, A., Suriya, N., Suwankitti, W. and Sangin, P. 2017. Fungal chitosan for growth and PAL gene expression of Dendrobium Sonia 'Bom 17' in vitro. Acta Hort. (ISHS) 1167:331-338
chitin, stress, gene expression, orchid, tissue culture