Establishing a protocol for dedifferentiation and redifferentiation of Tanacetum cinerariifolium leaves
Calli were induced from leaf fragments of pyrethrum plants grown from seeds of Japan origin on Murashige-Skoog (MS) medium supplemented with auxins (1‑naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)) and a cytokinin (6‑benzylaminopurine (BAP)). The hormone concentrations were optimized to yield green and healthy calli. Green spots on such calli were transferred to auxin-free and hormone-free medium successively to redifferentiate leaves. Pyrethrin contents were markedly reduced to below detectable levels by dedifferentiation.
Sakamori, K. and Matsuda, K. 2017. Establishing a protocol for dedifferentiation and redifferentiation of Tanacetum cinerariifolium leaves. Acta Hort. (ISHS) 1169:151-156
pyrethrins, callus, Tanacetum cineraiifolium, wound-induced volatiles