In vitro induction of polyploidy by colchicine treatment in herbaceous peony (Paeonia lactiflora Pall.)
Herbaceous peony is one of the most famous traditional flowers in China, with more than 4000 years of cultivation history. However, most peonies are diploid. The present study aimed at exploring an effective way to produce polyploidy using seeds or buds from three herbaceous peony cultivars ('Fen Yu Nu', 'Zhu Sha Pan' and 'Zhong Sheng Fen') by colchicine treatment. For seeds, NaClO (4%) solution was a more suitable disinfectant than HgCl2 (0.1%) solution, and the optimum disinfection time was 12 min. The best induction medium for 'Zhu Sha Pan' was 1/2 MS + 0.5 mg L-1 6‑BA + 0.2 mg L-1 GA3. Three chimeras were obtained from explants of 'Fen Yu Nu', at a rate of 7.14%, when seeds were first immersed in 0.1% colchicine solution for 12 h and then zygotic embryos were transferred to culture medium. For underground buds, HgCl2 (0.1%) solution was a more suitable disinfectant than NaClO (4%) solution, and the optimum disinfection time was 10 min. The best induction medium for 'Zhong Sheng Fen' was 1/2 MS + 1.0 mg L-1 6-BA + 0.2 mg L-1 IAA. Two tetraploids were produced from 'Zhu Sha Pan', at a rate of 6.67%, by using a mixed culture method (bud tips cultured in medium containing 0.2% colchicine for a week, then transferred to a medium free of colchicine). The polyploidy mutation rates of the control group and the other treatments were zero.
Wei, D.X., Tang, Z.J., Teixeira da Silva, J.A. and Yu, X.N. 2017. In vitro induction of polyploidy by colchicine treatment in herbaceous peony (Paeonia lactiflora Pall.). Acta Hort. (ISHS) 1171:265-278
tissue culture, polyploidy breeding, seeds, underground buds, disinfection ways