Bulblet regeneration in vitro from bulb-scales of Fritillaria thunbergii
Fritillaria thunbergii (Liliaceae), commonly known in Chinese as zhebei, is a perennial plant native to China with important medicinal and ornamental value. Wild populations are at a risk of rapid eradication, and are now very difficult to find. In order to protect wild resources and to set up an efficient system of rapid propagation, sections of bulb-scales from the vegetative period were used to regenerate bulblets in vitro. Surface-disinfected sections were cultured in Murashige and Skoog (MS) medium containing 35 g L-1 sucrose and supplemented with different types, concentrations and combinations of plant growth regulators [α-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KT)]. Bulblets were successfully induced from scale sections after 12 weeks of culture via two pathways. In the first pathway, callus was induced within 5 weeks from scale sections on solid MS medium that was supplemented with 0.5 mg L-1 BA and 2.0 mg L-1 NAA. After placing callus at low temperature (4°C) for 3 weeks, bulblets developed on the same medium within 4 weeks (11.3 bulblets explant-1). In the second (less effective) pathway, bulblets could be induced directly from scale sections on solid MS medium supplemented with 2.0 mg L-1 BA and 0.5 mg L-1 NAA, or 1.0 mg L-1 BA and 0.1 mg L-1 2,4-D, resulting in 8.2 and 7.8 bulblets explant-1, respectively. This is a simple but effective protocol for the mass propagation of F. thunbergii.
Hao, L.H., Teixeira da Silva, J.A. and Yu, X.N. 2017. Bulblet regeneration in vitro from bulb-scales of Fritillaria thunbergii. Acta Hort. (ISHS) 1171:25-30
fritillaries, traditional Chinese medicine, tissue culture, organ culture, micropropagation, conservation