Genetic structure of cultivated Latvian sea buckthorn (Hippophaë rhamnoides L.) germplasm revealed by molecular markers
Sea buckthorn (Hippophaë rhamnoides L.) is an interesting and promising crop due to its high content of valuable nutrients and bioactive substances. Increased growing of sea buckthorn stimulates the interest in breeding different sea buckthorn cultivars for fruit production as well as research and introduction of new plant material. Recently, some problems with pests and diseases have been identified. New germplasm, preferably of different origins, is therefore needed for breeding programs. This germplasm should be identified and selected by application of molecular markers (e.g., SSR and RAPD) to ensure sufficient genetic diversity. Unfortunately, such analyses are not always able to reveal the genetic origin of the plant material. Therefore six previously used polymorphic chloroplast SSR markers (cpSSR) were applied to characterize 33 accessions grown in Latvia, and developed from crosses among three subspecies of H. rhamnoides, namely ssp. mongolica, ssp. rhamnoides and ssp. fluviatilis. Although only three of the used cpSSR markers showed polymorphism in the investigated material, a grouping according to putative origin was achieved, and some samples with unknown origin were tentatively classified. In combination with eight SSR and sixteen RAPD markers, the cpSSR markers allowed complete discrimination of the tested sea buckthorn accessions, as well as determination of genetic structure in the Latvian sea buckthorn germplasm.
Lācis, G., Kota-Dombrovska, I and Bartulsons, T. 2017. Genetic structure of cultivated Latvian sea buckthorn (Hippophaë rhamnoides L.) germplasm revealed by molecular markers. Acta Hort. (ISHS) 1172:205-212
diversity, germplasm, microsatellites, RAPD, cpSSR