Characterization and clonal selection of Berberis microphylla G. Forst in the Chilean Patagonia Region for natural colorant purposes
In the food industry, the demand for natural colorants had been increasing in the last years because the concern regarding to synthetic colorant effects in human health. While the global natural colorant market reached US$720 million in 2013, it is expected to reach US$ 980 million in 2018. Global attention to anthocyanins has increased due also to their antioxidant properties. Calafate-berry (Berberis microphylla G. Forst) is an evergreen patagonian wild berry with high anthocyanin content, distributed in Chile from Curicó (34°59'0 S) to Tierra del Fuego (53°28'33 S), also in Argentine. A preliminary study of B. microphylla G. Forst was conducted in the Magellan's region in order to evaluate the phenotypic diversity for functional purposes. Over 40 accessions were selected in the field, and 21 evaluated for phenological stages, fruit yield, fruit size, seed fruit-1, total anthocyanin concentration analyzed by absorbance and anthocyanin classes analyzed by HPLC. Also genetic diversity was assayed using 12 selected SSRs. The results were analyzed by principal component analysis (PCA) and descriptive statistical analysis. In addition to the genetic diversity, significant differences (pKLEINERDAN0.05) were observed in most evaluated traits, being the fruit size and anthocyanin concentration the most determinant for further selection. The best clones were selected as parental lines for a further breeding program in Berberis species.
Pino, M.T., Mc Leod, C., Ojeda, A., Zamora, O. and Saavedra, J. 2017. Characterization and clonal selection of Berberis microphylla G. Forst in the Chilean Patagonia Region for natural colorant purposes. Acta Hort. (ISHS) 1172:249-254
Calafate-berry, wild-berry, total soluble sugars, anthocyanins, delphinidins