Development of high-throughput microsatellite markers for Korean pears using next generation sequencing
In this study, more than 145.4, 169.8, and 145.8 million sequencing reads were obtained from 'Cheongsilri', 'Whangkeumbae' and 'Minibae', respectively. They contained 66,748, 55,735, and 55,892 repeat motifs, with di-nucleotide (85.98, 86.04 and 86.02%) motifs as the most abundant repeat type, followed by tri- (11.24, 11.12, and 11.06%), tetra- (2.18, 2.23, and 2.21%), penta- (0.42, 0.42 and 0.42%), and hexa-nucleotides (0.17, 0.19 and 0.16%), respectively. Polymorphic SSR validations between samples were 1,674 in 'Cheongsilri' and 'Whangkeumbae', 1,509 in 'Cheongsilri' and 'Minibae' and 1,284 in 'Whangkeumbae' and 'Minibae'.
Kim, D., Oh, Y., Shin, H., Oh, S.I., Han, H., Won, J., Oh, S. and Kim, Y. 2017. Development of high-throughput microsatellite markers for Korean pears using next generation sequencing. Acta Hort. (ISHS) 1172:279-282
Pyrus spp., Asian pear, SSR