Transcriptomic and phytohormonal analysis of the interaction between Plum pox virus (PPV, sharka) and Prunus species
Plum pox virus (PPV, sharka disease) is the most important limiting factor for Prunus production in those affected areas. However, despite the fact that resistance to PPV has been studied for many years in these species at the genetic and genomic level, studies concerning the molecular basis of the expression of this resistance are scarcer. In this study, we integrate data from the gene expression changes in leaves from two apricot and one peach genotypes, susceptible and resistant to PPV, following virus infection using massive RNA sequencing (RNA-Seq) and the evaluation of phytohormonal patterns in the different treatments assayed. Transcriptomic differences at gene expression level confirmed that susceptibility to PPV in apricot and peach is a complex process based on a continuous battle between the virus (PPV) and the plant at pathogen resistance gene level and gene silencing level suppressed by the virus. On the other hand, resistance to PPV in apricot is also a complex process where MATH genes (control long distance movement of virus) are involved but also other genes (effect of genetic background). This resistant response observed in apricot is different to the response observed in the hypersensitive resistant plums. Phytohormonal analysis highlights the important implication of salycilic acid (SA) in the infection of the virus in Prunus. SA may also be related to gene silencing response of the plant supressed in the susceptible genotypes by PPV.
Rubio, M., Olivares, P.M., Batnini, M.A., Dicenta, F. and Martínez-Gómez, P. 2017. Transcriptomic and phytohormonal analysis of the interaction between Plum pox virus (PPV, sharka) and Prunus species. Acta Hort. (ISHS) 1172:295-298
PPV, sharka, Prunus, pathogenesis, symptoms, RNA-Seq, phytohormones