Intraspecific differentiation of Tunisian Ficus carica L. based on in silico atpB-rbcL PCR-RFLP fingerprinting
Variation of cytoplasmic genome offers tremendous opportunities to evaluate the level as well the structuration of molecular polymorphism in several fruit trees. The purpose of the current study is to evaluate the molecular diversity of atpB-rbcL spacer sequences of chloroplast DNA of Ficus carica cultivars. The atpB-rbcL spacer was sequenced for thirty-one fig cultivars. Sequences were submitted to NCBI GenBank and analyzed with different statistical methods. Hundred seventeen in silico enzymes were tested and only 46 had digested at the atpB-rbcL intergenic spacer. These have generated polymorphic restriction profiles. A total of 93 restriction sites were detected with 92 that were polymorphic. A genetic distance shows the presence of a large diversity between figs. The topology of the neighbor-joining dendrogram and the principal components analysis demonstrates no distinction between female trees and pollinators. The results suggest the existence of a common genetic basis between the two types of figs and show that the PCR-RFLP technique is a powerful technique and informative approach to explore cytoplasmic polymorphism of Tunisian fig trees. The expansion of this study to other regions of the genome and a higher number of cultivars would allow obtaining new molecular markers that could better appreciate the structure of the molecular diversity and organization within the local fig germplasm.
Baraket, G., Ben Abdelkrim, A., Haffar, S. and Salhi Hannachi , A. 2017. Intraspecific differentiation of Tunisian Ficus carica L. based on in silico atpB-rbcL PCR-RFLP fingerprinting. Acta Hort. (ISHS) 1173:63-68
Ficus carica L., atpB-rbcL spacer, PCR-RFLP, cytoplasmic polymorphism