Multivariate analysis of molecular and morphological diversity in fig (Ficus carica L.)

M. Aradhya, J.E. Preece, D. Velasco
Genetic polymorphism across 15 microsatellite loci among 194 fig accessions including Common, Smyrna, San Pedro, and Caprifig were analyzed using a cluster analysis (CA) and principal components analysis (PCA). The collection was moderately variable with observed number of alleles per locus ranging from four in loci MFC4, LMFC14, LMFC22, LMFC31, and LMFC35 to nine for LMFC30 with an average of 4.9 alleles per locus. Of nine loci exhibiting deviation of heterozygotes, three showed excess and six deficiency. However, mean heterozygosity across loci conformed to panmixia. Comparison of multilocus genotypes revealed many instances of synonymy or misidentification. The CA of molecular data revealed three groups differing significantly for frequency and composition of alleles for different loci. The PCA confirmed the results of CA. The CA and PCA of 103 accessions based on 24 categorical variables (vegetative, reproductive, and developmental traits) showed relationships somewhat incongruent with molecular analyses mainly in the placement of Caprifig but also other fig types. Weak genetic structure observed in the present study is probably due to genetic variability circulating across different fig types through dynamic gene flow facilitated by complex pollination interactions involving the symbiotic relationship between the fig and its pollinator.
Aradhya, M., Preece, J.E. and Velasco, D. 2017. Multivariate analysis of molecular and morphological diversity in fig (Ficus carica L.). Acta Hort. (ISHS) 1173:23-28
caprifig, genetic diversity, genetic structure, microsatellite, San Pedro, Smyrna

Acta Horticulturae