Transmission of fig mosaic disease agents to fig 'Sarılop' by Aceria ficus in Aydın, Turkey
Fig (Ficus carica) which leaves showing typical fig mosaic symptoms is infected by many different viruses. Fig mosaic diseases are transmitted by graft and vectors in nature. Fig leaf mottle associated virus 1,2 - FLMaV1,2; Fig mosaic virus - FMV; Fig mosaic associated virus 1,2 - FMaV-1,2; Fig badnavirus 1-FBV-1 are most common seen on fig orchard in Turkey. It is known that Fig mosaic virus (FMV) is transmitted by Aceria ficus but the other fig viruses transmission is unknown. In this study, we planned that transmission Fig mosaic viruses by Aceria ficus. Firstly, infected figs 'Sarılop' by A. ficus and healhty fig seedlings were analysed by One-step RT-PCR using 12 set of specific primers of the fig mosaic viruses. FLMaV-1, FMV and FaMV-1 was detected on donor (infected) figs whereas no amplification was detected on healthy fig seedlings. Donor fig leaves were cut under a stereo-microscope into small pieces. Every small pieces of fig leaves whıch have 10 adult A. ficus were transferred onto healthy fig seedlings. Transmission trials were performed on every two replications in two years. Eriophyid mites-exposed seedlings were checked weekly and observed symptoms. After transmission, fig seedlings leaves showed small leaf deformations, vein bandings yellowish spots after 20-60 days. Eriophyid mites-exposed seedlings and control plants were analyzed by One-step RT-PCR using 12 set of specific primers. FMV and FMaV-1 were determined by RT-PCR on the transmitted plant whereas no amplification was detected on control plants. According to these results, A. ficus appears to be able to transmit FMV and FMaV-1, but wasn't transmitted FLMaV-1 from ınfected fig 'Sarılop' to fig seedling in Aydın, Turkey.
Yorgancı, S. and Acıkgöz, S. 2017. Transmission of fig mosaic disease agents to fig 'Sarılop' by Aceria ficus in Aydın, Turkey. Acta Hort. (ISHS) 1173:279-284
fig mosaic disease, Aceria ficus, RT-PCR, FMaV-1, transmission