ASSESSMENT OF GENETIC DIVERSITY AMONG DACTYLIS GLOMERATA L. AS DETERMINED BY RAPD AND SRAP
SRAP (Sequence-related amplified polymorphism) and RAPD (Random Amplified Polymorphic DNA) are two advanced molecular markers for genetic research in turfgrass. The present study represented the first report that evaluated the levels and patterns of genetic diversity among 45 accessions of Dactylis glomerata L obtained from eight geographically and environmentally distinct countries and regions. Twenty-one pairs of primer were used and 480 bands were produced in SRAP, of which 405 (84.38%) were polymorphic. On the other hand, twenty primers were used and 120 bands were produced in RAPD, of which 97 (80.83%) were polymorphic. The genetic similarity coefficients varied from 0.6248-0.9686 and 0.6154-0.9254, respectively. The genetic diversity of D. glomerata L. from China and America was richer than others. Based on cluster and principal component analysis on the genetic characteristics, all collections could be divided into four groups and five groups in two markers, respectively. Accessions from the identical continent were classified into the same group, indicating the geographical distribution of genetic diversity of D. glomerata L., which suggested that the genetic basis of cultivars is narrow, especially that in Chinese cultivars. The collection from Australia was different from other accessions in genetic diversity. Based on these results, SRAP and ISSR are considered useful tools for evaluating the genetic diversity for D. glomerata L., but SRAP detected more variance and gave clearer cluster groups. According to the analysis of genetic diversity and relationships, the appropriate strategies for collection and conservation of germplasm resources also were discussed.
Bing Zeng , , Xin-Quan Zhang , and Ying Lan , 2008. ASSESSMENT OF GENETIC DIVERSITY AMONG DACTYLIS GLOMERATA L. AS DETERMINED BY RAPD AND SRAP. Acta Hort. (ISHS) 783:291-302
Dactylis glomerata L., genetic diversity, germplasm, RAPD, SRAP