EXPRESSION OF REFERENCE GENES IN NODOSITIES AND THEIR APPLICATION
As the grape phylloxera (Daktulosphaira vitifoliae Fitch), a devastating pest in viticulture gains in importance once again, there is an urgent need to understand the phylloxera-root interaction in more detail. One possibility to expand the knowledge is by applying a molecular method such as quantitative real time polymerase chain reaction (qRT-PCR). This technology allows the relative quantification of gene expression among different tissues. As reference genes which are required for normalizing gene expression are not always equally expressed in similar tissues and environmental conditions, this study aims to identify the two reference genes that are most stably expressed in grapevine roots and root galls (nodosities) caused by phylloxera. Our results reveal that ubiquitin 1 (UBI1) and elongation factor 1α (EF1) can be recommended as reference genes in further gene expression studies evaluating this interaction. To implement our approach a qRT-PCR study was performed testing different putative expansins in V. vinifera identified through sequence homology with genes described in Arabidopsis thaliana.
Lawo, N.C., Maleno, F., Griesser, M. and Forneck, A. 2011. EXPRESSION OF REFERENCE GENES IN NODOSITIES AND THEIR APPLICATION. Acta Hort. (ISHS) 904:77-84
phylloxera, grapevine, root, UBI1, EF1, RT-PCR