EFFECT OF DIFFERENT LIGHT AND TWO POLYSACCHARIDES ON THE PROLIFERATION OF PROTOCORM-LIKE BODIES OF CYMBIDIUM CULTURED IN VITRO
Light is one of the most important abiotic factors that influences the successful establishment and subsequent development of a plant culture. The aim of this study was to examine the effect of light quality (white, red, blue and green) and two polysaccharides; chitosan (Chitosan H) and hyaluronic acid (HA9) on proliferation of the protocorm-like bodies (PLBs) of in vitro grown Cymbidium. Chitosan H and HA9 have of recent been suggested as important components of Cymbidium in vitro culture media. The highest PLBs formation, shoot formation rate (90%) and root formation rate (50%) were found amongst explants cultured on medium supplemented with 0.1 mg/L Chitosan H under green light. After 11 weeks of culture, fresh weight of PLBs was higher (241.3 mg) at HA9 (1 mg/L) treatment with green light. The average number of PLBs (5.7) was higher under green light at HA9 treatment. PLBs under white light showed the highest number of shoot (1.2) at Chitosan H treatment. These results suggest that this newly developed light source could be used as an energy efficient light source for the propagation of Cymbidium PLBs in vitro and also green light plays an important role of PLBs, shoot and root formation.
Nahar , S.J., Shimasaki, K. and Haque, S.M. 2012. EFFECT OF DIFFERENT LIGHT AND TWO POLYSACCHARIDES ON THE PROLIFERATION OF PROTOCORM-LIKE BODIES OF CYMBIDIUM CULTURED IN VITRO. Acta Hort. (ISHS) 956:307-313
chitosan, energy efficient light source, hyaluronic acid, LED, PLBs