UTILIZATION OF DATE WASTES AS A SUBSTRATE FOR THE PRODUCTION OF CITRIC ACID AND α-AMYLASE
The present study deals with submerged citric acid and α-amylase fermentation by Aspergillus niger using date wastes as the basal fermentation medium. Effects of process variables on production of citric acid and α-amylase were studied, and accordingly optimum conditions have been determined. At this effect, the cumulative effect of fermentation period (144 h), sugar concentration (150.0 g/L), initial pH (3.5), methanol concentration (3%), ammonium nitrate concentration (2.5 g/L) and potassium phosphate concentration (2.5 g/L) during the fermentation process of date wastes did increase the citric acid production up to 126.0 g/L. On the other hand, the maximum α-amylase activity i.e., 2716.66 µmol/L/min was obtained under optimum conditions of an incubation period of 96 h, temperature of 30°C, initial pH of 5.5, sugars concentration of 20 g/L and utilisation of yeast extract as nitrogen source.
Acourene, S., Djafri , K., Djidda, A. and Amourache, L. 2013. UTILIZATION OF DATE WASTES AS A SUBSTRATE FOR THE PRODUCTION OF CITRIC ACID AND α-AMYLASE. Acta Hort. (ISHS) 994:135-149
submerged fermentation, Aspergillus niger, temperature, pH, sugars, methanol