EXPLOITATION OF AFLP AND RAMPO MARKERS IN THE ASSESSMENT OF GENETIC DIVERSITY WITHIN TUNISIAN DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS
Tunisian date-palm germplasm is characterized by an important genetic diversity since more than 250 ecotypes have been identified in the continental and coastal oasises of the country. However, these important phytogenetic resources are threatened either by severe genetic erosion or plagues. In order to molecularly characterize the local germplasm, we have been interested by the development of Random Amplified Microsatellite Polymorphism (RAMPO) and Amplified Fragment Length Polymorphism (AFLP) methods to generate molecular markers. Our results showed that using both markers systems, the Tunisian date-palm germplasm is characterized by a large and continuous genetic diversity. In addition and due to the greater number of markers per assay, AFLP technique seems to be more informative than RAMPO method. In fact, 186 and 428 polymorphic bands were detected using RAMPO and AFLP primers, respectively. Moreover, AFLP markers were found most polymorphic with the highest average PIC value (0.7). In addition, independent and combined cluster analyses of the RAMPO and AFLP fragments showed that cultivars are clustered independently from both trees sex and geographical origin.
Rhouma-Chatti, S., Zehdi-Azzouzi, S., Dakhlaoui-Dkhil, S., Chérif , E. and Trifi, M. 2013. EXPLOITATION OF AFLP AND RAMPO MARKERS IN THE ASSESSMENT OF GENETIC DIVERSITY WITHIN TUNISIAN DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARS. Acta Hort. (ISHS) 994:231-240
coastal oasis, germplasm, ecotypes, molecular markers, phytogenetic resource