EFFECT OF VARIOUS LEVELS OF SUCROSE AND NITROGEN SALTS ON THE GROWTH AND DEVELOPMENT OF LILY BULBLETS IN VITRO
Among the many environmental factors, carbon (C) and nitrogen (N) are crucial for plants to perform the fundamental cellular activities, and the metabolisms of the two elements are intimately linked. Current knowledge indicates that carbon and nitrogen are two signals that influence plant growth and development. The aim of the study was to investigate the influence of sucrose (20, 40, 60, 80 g L-1) and nitrogen salts KNO3, NH4NO3 (25, 50 and 100% of standard MS strength), and their interaction on the growth and development of lily bulblets (Lilium Robina, L. martagon var. album) in vitro. Increased sucrose levels significantly enhanced the average fresh weight of bulblets, but inhibited the emergence and growth of leaves, especially in the presence of low strengths of the nitrogen salts. At all levels of sucrose, the increased supply of the nitrogen salts in the culture medium significantly enhanced the growth of bulblets and emergence of leaves. The highest fresh weight of bulblets was found on the media with high sucrose levels (60 and 80 g L-1) and with 100% (for cultivar Robina) or 50% (for L. martagon var. album) strength of KNO3 and NH4NO3. There is clearly an interaction between sucrose and nitrogen salts in the growth and formation of lily bulblets. A high level of sucrose and the nitrogen salts in the medium strongly promoted bulblet fresh weight. In contrast, a medium with a high sucrose to nitrogen ratio had an inhibitory effect on leaf formation.
Gabryszewska, E. and Sochacki, D. (2013). EFFECT OF VARIOUS LEVELS OF SUCROSE AND NITROGEN SALTS ON THE GROWTH AND DEVELOPMENT OF LILY BULBLETS IN VITRO. Acta Hortic. 1002, 139-145
Lilium, bulblet growth, nitrogen, sucrose, micropropagation