DETECTION OF VIRUSES INFECTING LILIUM SPP. BY RT-PCR AND REAL-TIME PCR

B. Nesi, S. Lazzereschi, S. Pecchioli, A. Grassotti, D. Rizzo, L. Stefani, M. Paoli, M. Della Bartola, A. Materazzi
In order to enhance the Italian lily bulb production, a breeding program was carried out at CRA-VIV in Pescia (PT – Italy) during the last years. Asiatic hybrids (Lilium × elegans Thunb.), lily cultivars and other native species were involved in the program. The obtained lily lines, which presented interesting traits, were preserved in a collection. After years of vegetative propagation, some of the new selections showed symptoms referable to viral infections. Virus diseases represent some of the most dangerous threats of Lilium, so the application of fast and effective diagnostic techniques for early detection is very important. The aim of the present study, in the frame of a phytosanitary survey of the lily collection, is to investigate the presence and incidence of Cucumber mosaic virus (CMV), Lilium symptomless virus (LSV), Lily mottle virus (LMoV) and two tospoviruses (Impatiens necrotic spot virus, INSV, and Tomato spotted wilt virus, TSWV). Among the 60 samples object of this study, infections by LSV and CMV were frequently observed. Also LMoV was detected in a smaller amount of samples. All of the samples were negative to INSV and TSWV.
Nesi, B., Lazzereschi, S., Pecchioli, S., Grassotti, A., Rizzo, D., Stefani, L., Paoli, M., Della Bartola, M. and Materazzi, A. (2013). DETECTION OF VIRUSES INFECTING LILIUM SPP. BY RT-PCR AND REAL-TIME PCR. Acta Hortic. 1002, 215-221
DOI: 10.17660/ActaHortic.2013.1002.26
https://doi.org/10.17660/ActaHortic.2013.1002.26
Lilium, RT-PCR, Real Time PCR, CMV, LSV, LMoV, INSV, TSWV
English

Acta Horticulturae