S. Lazzereschi, B. Nesi, S. Pecchioli , A. Grassotti, D. Rizzo, L. Stefani , M. Paoli
Calla lily (Zantedeschia aethiopica, Araceae Family) has become one of the most popular cut flowers worldwide for its ornamental characteristics, such as spathe beauty and postharvest shelf-life. Calla lily is mainly used as cut flower, but also as potted plant. Tospovirus (Bunyaviridae Family) causes severe agronomic and economic damages to various crops and flowers, one of these is Impatiens necrotic spot virus (INSV); it mainly affects a lot of ornamental plants, i.e., cineraria, cyclamen, prairie gentian and many others, including perennials. INSV causes yellow or brown ring spots, round brown black or white spots, brown or black stem sections, black or brown necrosis at the leaf base, stunting, etc. In calla lily the effects of infection are chlorotic or yellow spots radiating from the midrib towards the edge of the leaf. ELISA is a routine method for diagnosis of plant viruses including Tospoviruses and RT-PCR has been widely used as a highly sensitive and specific detection method. In the present work some of calla lily plants maintained at CRA-VIV were analyzed in collaboration with the phytopatological laboratory of Tuscany Region. INSV was detected directly in the field by the immune-cromatographic ‘Lateral Flow’ technique and a protocol for double step RT-PCR was performed, using different amplification profiles. The detection of INSV can be reliable also by the ‘Lateral Flow’ technique, but the results need to be confirmed by RT-PCR.
Lazzereschi, S., Nesi, B., Pecchioli , S., Grassotti, A., Rizzo, D., Stefani , L. and Paoli , M. (2013). SERIOUS DAMAGES BY IMPATIENS NECROTIC SPOT VIRUS IN ZANTEDESCHIA AETHIOPICA. Acta Hortic. 1002, 319-325
DOI: 10.17660/ActaHortic.2013.1002.42
calla lily, INSV, ELISA test, 'Lateral Flow' technique, RT-PCR

Acta Horticulturae