MOLECULAR CHARACTERIZATION OF PORTUGUESE ISOLATES OF LEPTOSPHAERIA MACULANS USING PCR-ISSR AND RAPD MARKERS
There are scarce information regarding Leptosphaeria maculans isolates from Portugal. This paper reports the first molecular study with Portuguese L. maculans isolates. PCR-ISSR and RAPD markers were used to study the genetic relationships of 18 Portuguese isolates of Leptosphaeria maculans from Beja region (south Portugal) to 12 other reference isolates representative of each group of the L. maculans species complex. Cluster and principal components analyses were conducted using PCR-ISSR and RAPD data from amplification with selected ISSR primers and RAPD markers, detecting 234 polymorphic fragments. The results showed that the 30 isolates clustered into two distinct groups (Tox+ and Tox0 isolates) and 4 subgroups: i) a large and compact subgroup containing all the Tox+ L. maculans brassicae isolates including all Portuguese isolates; ii) the unique Lepidium isolate; iii) a relatively heterogeneous subgroup with the Tox0 NA2 L. biglobosa canadensis isolates; and iv) a dispersed subgroup with the other Tox0 NA1 and NA3 L. biglobosa isolates and NA2 L. biglobosa erysimii isolate. There is low similarity between these three isolates. The Portuguese and foreign Tox+ L. maculans brassicae isolates could be further divided into phenetic groupings/clusters. These groupings did not correspond to their pathogenicity groups revealed by plant differentials. The Portuguese L. maculans population do not seem to be different to that occurring in other countries (Australia, France, Germany, UK) which confirms that there is a large-scale mixing in L. maculans complex.
Godinho , J.S., Dias, J.S., Balesdent , M.H. and Mendes-Pereira, E. (2013). MOLECULAR CHARACTERIZATION OF PORTUGUESE ISOLATES OF LEPTOSPHAERIA MACULANS USING PCR-ISSR AND RAPD MARKERS. Acta Hortic. 1005, 573-580
Phoma lingam, L. biglobosa, isolate grouping, numerical taxonomy, molecular markers, PCR-ISSR, RAPD