ISOLATION AND CHARACTERISATION OF RESISTANT GENE ANALOGUES FROM GUAVA

A.A. Severn-Ellis, M. Schoeman, S. Willemse , A. Sippel
The South African Guava industry has shown a steady increase in production over the past five years driven by an increased global demand for guava juice and puree. Renewed outbreaks of guava wilt disease in the Limpopo and Mpumalanga provinces also affecting the tolerant cultivar ‘TS-G2’, is placing the guava industry under threat once again. ‘TS-G2’ was successfully used in replacing many of the production areas previously lost due to GWD. No suitable alternative control measures are currently available thereby emphasizing the importance of the development of wilt resistant guava cultivar(s). Comparative analyses of the R-genes, from divergent plants, that provide resistance to a variety of pathogens have revealed certain conserved amino acid domains. These domains include nucleotide-binding sites (NBS), leucine-rich repeat (LRR), trans-membrane domains (TM) and serine/threonine protein kinases (PKs) arranged in various combinations. Degenerate PCR primers designed to these conserved domains were employed in the isolation of resistance gene candidates (RGCs) from guava. The isolation and identification of possible resistance genes reported in this study represents the first step towards the development of GWD resistant guava cultivars.
Severn-Ellis, A.A., Schoeman, M., Willemse , S. and Sippel, A. (2013). ISOLATION AND CHARACTERISATION OF RESISTANT GENE ANALOGUES FROM GUAVA . Acta Hortic. 1007, 699-707
DOI: 10.17660/ActaHortic.2013.1007.81
https://doi.org/10.17660/ActaHortic.2013.1007.81
resistance gene analogues, guava wilt disease
English

Acta Horticulturae