DNA POLYMORPHISM IN ETHYLENE SYNTHETIC GENES IN THAI LOCAL AND COMMERCIAL PAPAYA CULTIVARS
Papaya (Carica papaya L.) is an important tropical fruit crop for consumption and as an ingredient in food and cosmetics products. Papaya is a climacteric fruit and thus ethylene plays a key role in ripening. DNA polymorphism of three genes in ethylene synthetic pathway including S-adenosyl methionine (SAM) synthase, 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase in 29 local and commercial papaya samples was investigated. Gene specific primers were designed for each of ethylene synthetic genes and used to amplify DNA fragments with approximately size of 161-484 base pairs. The results showed polymorphic bands of DNA fragments amplified from all three ethylene synthetic genes. Neighbor Joining (NJ) analysis indicated that Thai local and commercial cultivars assembled closely into cluster I while the rest was loosely grouped into cluster II.
Burns, P., Phuangrat, B., Nacharoen, S., Siripanich, J. and Wasi, S. (2014). DNA POLYMORPHISM IN ETHYLENE SYNTHETIC GENES IN THAI LOCAL AND COMMERCIAL PAPAYA CULTIVARS. Acta Hortic. 1022, 79-84
Carica papaya, DNA polymorphism, ethylene synthetic pathway