ANTIMICROBIAL ACTIVITY OF IN SITU FORMING GEL COMPRISING CORK TREE SEED EXTRACT
Cork tree (Sonneratia caseolaris) is a plant in the family of Sonneratiaceae whose extract has been reported for the traditional use as an astringent and an antiseptic. The methanolic seed extract of cork tree was employed as the active compound in the in situ forming gel containing 30% Eudragit RS in N-methyl pyrrolidone. The prepared gel exhibited the in situ formimg gel properties. The anitimicrobial activity was tested with agar diffusion method using Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8740, Candida albicans ATCC 17110, Streptococcus mutans ATCC 27175 and anaerobic bacteria from oral cavity as tested microbes. The obtained gel could inhibit all tested microbes. The release of three markers (gallic acid, luteolin glycoside and luteolin) from 10% w/w seed extract gel was performed using dialysis tube method in phosphate buffer pH 6.8. The released amount of three markers was analyzed simultaneously with HPLC method. Gallic acid and luteolin, respectively, were rapidly released in the initial stage and gradually released for 720 min. Luteolin glycoside started to release at 480 min. and lesser amount was released into the medium at 720 min. In situ forming gel comprising cork tree seed extract exhibited the potential dosage form to deliver the active compound from natural product and show antimicrobial activity.
Phaechamud, T., Mahadlek, J. and Charoenteeraboon, J. (2014). ANTIMICROBIAL ACTIVITY OF IN SITU FORMING GEL COMPRISING CORK TREE SEED EXTRACT . Acta Hortic. 1023, 53-58
methanolic seed extract, anaerobic bacteria, gallic acid, luteolin