SIMPLE SEQUENCE REPEATS (SSR) PROFILING OF CULTIVATED 'LIMAU PURUT' IN MALAYSIA

G.P.S. Elcy, Y.J. Park, M.C. Mahani, M.N. Normah
In Malaysia, ‘Limau Purut’ (Citrus hystrix) is mainly grown in home gardens and nurseries. There is potential for C. hystrix to be grown commercially but there is little genetic information to assist in selection of cultivars. In this study, the genetic variability and genetic relatedness among plants cultivated in the different states of peninsular Malaysia were assessed using simple sequence repeat (SSRs). Thirty pairs of SSR primers derived from C. unshiu were screened and 20 SSR primer pairs were utilized to assess genetic variability and relatedness among 100 samples. The percentage of SSR transferability from C. unshiu to C. hystrix was 66.67% which indicated that the primer sequences flanking simple sequence repeats were conserved between these two Citrus species. Low allelic diversity was found with an average of 2.9 alleles per locus. Polymorphic information content (PIC) ranged from 0 to 0.710. Based on UPGMA clustering analysis, two distinct groups were observed with mean genetic distance of 0.228. Samples collected from the citrus germplasm collection at MARDI clustered separately from the remaining samples collected from orchards, indicating derivation from possibly two distinct cultivars. Low genetic variability within each group was probably due to vegetative propagation and/or inability to detect differences among samples using these primers. Correlation of molecular markers with phenotypic characteristics will be useful in selection of cultivars for commercial cultivation and conservation purposes.
Elcy, G.P.S., Park, Y.J., Mahani, M.C. and Normah, M.N. (2014). SIMPLE SEQUENCE REPEATS (SSR) PROFILING OF CULTIVATED 'LIMAU PURUT' IN MALAYSIA. Acta Hortic. 1024, 77-84
DOI: 10.17660/ActaHortic.2014.1024.6
https://doi.org/10.17660/ActaHortic.2014.1024.6
microsatellites, genetic variability, genetic relatedness, probability of identity
English

Acta Horticulturae