HISTOLOGICAL ANALYSIS OF SOMATIC EMBRYOGENESIS INDUCED IN TROPICAL LADY'S SLIPPER ORCHID (PAPHIOPEDILUM NIVEUM)

P. Kaewubon , U. Meesawat
Indirect somatic embryogenesis, an asexual process of plant propagation, is an acceptable tool for cell differentiation and developmental studies. This study was conducted to elucidate the developmental pathway of a somatic embryo in the Paphiopedilum niveum orchid. The callus-derived somatic embryo was obtained by using modified Vacin and Went (VW) medium supplemented with 0.1 mg L-1 naphthalene acetic acid (NAA) and 0.5 mg L-1 thidiazuron (TDZ) as well as 10 g L-1 sucrose. Developmental phases were characterized by histological analysis. The callus-derived somatic embryos developed progressively through the early globular, globular and cotyledonary stages. After a month of culture, a proembryonic mass (or early globular-shape stage) originated on the surface of the callus and differentiated rapidly to a globular somatic embryo with a well-demarcated protoderm. These somatic embryos developed protrusions that indicated the cotyledonary stages after two months of culture. The organized structures of plantlets were eventually obtained when protocorm-like bodies (PLBs) involving somatic embryogenesis were transferred to modified Murashige and Skoog (MS) medium supplemented with 50 g L-1 homogenated banana. Histological analysis also revealed that the pattern of somatic embryo formation of P. niveum showed the same process as the developmental pathway of monocot species.
Kaewubon , P. and Meesawat, U. (2014). HISTOLOGICAL ANALYSIS OF SOMATIC EMBRYOGENESIS INDUCED IN TROPICAL LADY'S SLIPPER ORCHID (PAPHIOPEDILUM NIVEUM) . Acta Hortic. 1025, 231-236
DOI: 10.17660/ActaHortic.2014.1025.33
https://doi.org/10.17660/ActaHortic.2014.1025.33
cotyledonary stages, developmental pathway, proembryonic mass, protoderm, somatic embryo
English

Acta Horticulturae