EFFECT OF HYALURONIC ACID ON ORGANOGENESIS IN PROTOCORM-LIKE BODY (PLBS) OF SOME CYMBIDIUM SPECIES IN VITRO
Hyaluronic acid (HA), is a naturally occurring biopolymer, which serves important biological functions in bacteria and higher animals including humans. It is a component of the extra cellular matrixes of some tissues, can modulate growth factor and cytokine secretion, inhibit proteine hydrolysis and influence several cellular functions, such as adhesion, growth, migration, proliferation and differentiation. The present study was conducted to investigate the effect of hyaluronic acid on in vitro regulation of protocorm-like bodies (PLBs) of Cymbidium insigne and Cymbidium finlaysonianum. PLBs of Cymbidium species were explanted on modified MS medium supplemented with HA at various concentrations 0, 0.001, 0.01, 0.1, 1 and 10 mg L-1. New PLBs and shoots were successfully regenerated on modified MS medium supplemented with hyaluronic acid. In Cymbidium insigne, 0.01 mg L-1 HA induced a higher PLBs (80%) and shoots (60%) proliferation rate. In Cymbidium finlaysonianum, the highest PLBs induction rate was obtained with 0.001 mg L-1 HA (80%) but without shoot proliferation. The highest shoots (40%) were obtained at 0.01 mg L-1 hyaluronic acid. These results indicated that HA would work as a plant growth regulator that enhances both PLB and shoot formation.
Nahar , S.J. and Shimasaki, K. (2014). EFFECT OF HYALURONIC ACID ON ORGANOGENESIS IN PROTOCORM-LIKE BODY (PLBS) OF SOME CYMBIDIUM SPECIES IN VITRO. Acta Hortic. 1025, 237-242
biopolymer, plant growth regulators, regeneration, extra cellular matrixes, cytokine secretion