INTERSUBGENERIC HYBRIDIZATION BETWEEN PARACURCUMA AND EUCURCUMA VIA EMBRYO RESCUE
Interspecific hybrids between Paracurcuma (Curcuma alismatifolia and C. parviflora) and Eucurcuma (C. angustifolia and C. aurantiaca) subgenera were obtained by embryo rescue. Pre-fertilization barriers were investigated by observing pollen germination on the stigma surface and pollen tube growth in the pistil. A study on fruit setting revealed that the intersubgeneric crosses between Paracurcuma and Eucurcuma subgenera could set fruit only in direct crosses and producing only a few vigorous seeds per fruit. For the crosses that produced embryos, the 14-28 day-after-pollination (DAP) embryos were excised and cultured in vitro. The embryos obtained from 21 DAP fruits showed high shoot regeneration when cultured on MS medium containing 1 mg L-1 BA, 1 mg L-1 GA and 0.1 mg L-1 NAA. The regenerated hybrids between C. alismatifolia and C. aurantiaca, C. alismatifolia and C. angustifolia, and C. parviflora and C. aurantiaca showed intermediate characteristics between the parents.
Puangkrit, T. and Nontaswatsri, C. (2014). INTERSUBGENERIC HYBRIDIZATION BETWEEN PARACURCUMA AND EUCURCUMA VIA EMBRYO RESCUE . Acta Hortic. 1025, 37-42
interspecific hybrids, curcuma, pollen viability, pollen germination, embryo culture