H. Tasaki, H. Takagi, H. Otsubo, C. Hatakeyama, Y. Saito, E. Oka, J. Amano, D.S. Han , M. Nakano, Y. Watanabe
The liliaceous perennial plants, Tricyrtis spp., are native to East Asia. Some of these species are popular in Japan as ornamental plants for pot and garden uses because of their beautiful foliage, attractive flowers and the ability to grow in the shade. In order to widen horticultural variability and develop novel cultivars, interspecific pollination and subsequent ovule culture were carried out using T. flava and T. formosana ‘Edonohana’ and ‘Seiryu’. Two weeks after pollination, ovules with placental tissues were isolated from enlarged ovaries and cultured on half-strength MS medium without plant growth regulator. For T. flava  T. formosana ‘Edonohana’, five enlarged ovaries were obtained from nine pollinated flowers, and six seedlings were obtained among 405 cultured ovules. On the other hand, T. flava  T. formosana ‘Seiryu’, two enlarged ovaries were obtained among 12 pollinated flowers, and 4 seedlings were obtained among 118 cultured ovules. The hybridity of all the ten ovule culture-derived plants was confirmed by flow cytometry (FCM) and random amplified polymorphic DNA (RAPD) analyses. Most hybrid plants produced flowers three years after cultivation in the greenhouse. Hybrid plants showed novel morphological characteristics compared with their parents, some of which were horticulturally attractive. The results obtained in the present study indicated the validity of interspecific hybridization for breeding of Tricyrtis spp.
Tasaki, H., Takagi, H., Otsubo, H., Hatakeyama, C., Saito, Y., Oka, E., Amano, J., Han , D.S., Nakano, M. and Watanabe, Y. (2014). INTERSPECIFIC HYBRIDIZATION BETWEEN TRICYRTIS FLAVA AND T. FORMOSANA VIA OVULE CULTURE. Acta Hortic. 1025, 43-50
DOI: 10.17660/ActaHortic.2014.1025.5
embryo rescue, FCM analysis, liliaceous ornamental plant, RAPD analysis

Acta Horticulturae