CALLUS INDUCTION AND PLANT REGENERATION FROM IMMATURE COTYLEDON OF CAMELLIA AZALEA

J.Y. Li, Z.Q. Fan, B. Wu
Camellia azalea (C. changii) naturally grows in the very limited habitat of the E’huangzhang Natural Reserve of Yangchun, Guangdong Province, China, and it is the only species that blooms in the summer in the sect. Camellia of the genus Camellia. Thus it was widely used as a breeding material and a precious ornamental plant because no summer-flowering camellia species or cultivars have been found in the ornamental market. However, some local ornamental growers transplanted wild C. azalea plants to their nurseries and resulted in dramatic decrease in the number of wild individuals. A rapid propagation technique is needed to propagate and reserve this unique camellia species. This project was aimed to develop a tissue culture method on callus induction and plant regeneration with immature cotyledons of C. azalea cultured on MS medium supplemented with various combinations of plant growth regulators (PGRs). The results showed that an average of 76.15% cotyledons successfully developed callus on MS medium with 0.5 mg•L-1 2,4-D, 2.0 mg•L-1 6-BA and 50 ml L-1 coconut water (CW) . Differentiation capacity of the callus was well maintained by being subcultured on MS medium supplemented with 0.4 mg L-1 NAA, 2.0 mg•L-1 6-BA and 50 ml•L-1 CW for more than 45 days. Adventitious buds were then obtained after about 65 days on the medium containing 0.125 mg•L-1 NAA, 10.0 mg L-1 6-BA, 100 ml•L-1 CW, 500 mg•L-1 proline and 100 mg L-1 glutamine. The rate of differentiation was measured up to 91.85%, and was further increased to 97.02% on the medium added with 15 mg•L-1 PAA. About 80% adventitious buds developed their quality roots in about 50 days by using ‘Filter Paper Bridge Rooting Method’.
Li, J.Y., Fan, Z.Q. and Wu, B. (2014). CALLUS INDUCTION AND PLANT REGENERATION FROM IMMATURE COTYLEDON OF CAMELLIA AZALEA. Acta Hortic. 1035, 157-162
DOI: 10.17660/ActaHortic.2014.1035.18
https://doi.org/10.17660/ActaHortic.2014.1035.18
Camellia azalea (C. changii), callus induction, adventitious buds, plant regeneration
English
1035_18
157-162

Acta Horticulturae