THE DEVELOPMENT OF PLANT VITRIFICATION SOLUTION 2 AND RECENT PVS2-BASED VITRIFICATION PROTOCOLS
On October 5, 2012, Professor Akira Sakai passed away at the age of 92. He was an outstanding pioneer researcher of cryopreservation. Professor Sakai stressed that there was an urgent need to develop a relatively simple and efficient method of cryopreservation that could replace the conventional method, especially for storing the genetic resources of tropical plants. Using nucellar embryo cells of orange, he developed the glycerol-based Plant Vitrification Solution 2 (PVS2) in 1988 and, using wasabi meristems, an osmoprotective solution (LS) in 1992. The PVS2 vitrification protocol using these two solutions is considered an outstanding contribution to the advancement of cryopreservation research and of plant germplasm preservation worldwide. This methodology made possible the efficient cryopreservation of numerous plants species of temperate and tropical origin. Now more than 200 plant species have been successfully cryopreserved using this technique. The technique also has been further developed with new protocols such as encapsulation-vitrification, droplet-vitrification and vitrification using an aluminium plate. Professor Sakai was a distinguished teacher who guided with passion and consideration numerous Japanese and overseas scientists to their doctorate. He trained many scientists from around the world in cryopreservation techniques. His students would like to express their deep appreciation and respect for the inspiring leadership Professor Sakai provided. They will continue their research into cryopreservation methods of plants.
Matsumoto, T. and Niino, T. (2014). THE DEVELOPMENT OF PLANT VITRIFICATION SOLUTION 2 AND RECENT PVS2-BASED VITRIFICATION PROTOCOLS. Acta Hortic. 1039, 21-28
Akira Sakai, plant cryopreservation, PVS2, vitrification