EFFECTIVENESS OF CULTURE MEDIA ON THE SUCCESS OF EMBRYO RESCUE AMONG DIFFERENT CROSSES OF SEEDLESS GRAPES

Effects of culture media on the success of embryo rescue were tested among three different cross combinations of seedless grapes. ER, N&N, WP and ½ MS media were used. The best medium for ovule development was ER + 6-BA 0.5 mg/L among all 3 cross combinations. For embryo germination and plantlet formation, the success rate varied among different cross combinations. The best media for seedling establishment in ‘Flame Seedless’ × ‘Centennial’, for example, were 1/2 MS for embryo germination and 1/2 MS + IBA 0.1 mg/L for seedling growth. The best media for ‘Otilia’ × ‘Venus’ were 1/2 MS + IAA 0.1 mg/L for embryo germination, 1/2 MS + IAA 0.1 mg/L for seedling growth, and half strength B5 + IAA 0.125 mg/L for subculture, respectively. The best media for ‘Venus’ × ‘Himrod’ were 1/2 MS for embryo germination, WP + 6-BA 0.1 mg/L for seedling growth, half strength of B5 + IAA 0.125 mg/L for subculture, respectively.