EFFECTIVENESS OF CULTURE MEDIA ON THE SUCCESS OF EMBRYO RESCUE AMONG DIFFERENT CROSSES OF SEEDLESS GRAPES

Y. Hao, H. Wang, R. Yang , F.L. Wang, J. Lu
Effects of culture media on the success of embryo rescue were tested among three different cross combinations of seedless grapes. ER, N&N, WP and ½ MS media were used. The best medium for ovule development was ER + 6-BA 0.5 mg/L among all 3 cross combinations. For embryo germination and plantlet formation, the success rate varied among different cross combinations. The best media for seedling establishment in ‘Flame Seedless’ × ‘Centennial’, for example, were 1/2 MS for embryo germination and 1/2 MS + IBA 0.1 mg/L for seedling growth. The best media for ‘Otilia’ × ‘Venus’ were 1/2 MS + IAA 0.1 mg/L for embryo germination, 1/2 MS + IAA 0.1 mg/L for seedling growth, and half strength B5 + IAA 0.125 mg/L for subculture, respectively. The best media for ‘Venus’ × ‘Himrod’ were 1/2 MS for embryo germination, WP + 6-BA 0.1 mg/L for seedling growth, half strength of B5 + IAA 0.125 mg/L for subculture, respectively.
Hao, Y., Wang, H., Yang , R., Wang, F.L. and Lu, J. (2014). EFFECTIVENESS OF CULTURE MEDIA ON THE SUCCESS OF EMBRYO RESCUE AMONG DIFFERENT CROSSES OF SEEDLESS GRAPES. Acta Hortic. 1046, 259-263
DOI: 10.17660/ActaHortic.2014.1046.33
https://doi.org/10.17660/ActaHortic.2014.1046.33
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English

Acta Horticulturae