REGULATION OF ANTHOCYANIN BIOSYNTHESIS IN STRAWBERRY (FRAGARIA SP.) BY OVER-EXPRESSION OF A KEY TRANSCRIPTION FACTOR
Anthocyanins are secondary metabolites found in higher plants that contribute to the colours of fruits and leaves. Previous studies, in other species, have shown the regulation of anthocyanin biosynthesis is mainly at the level of transcriptional regulation of structural genes by transcription factors that include MYB, bHLH and WD-repeat proteins. Here we report that stable transformants of strawberry over-expressing 35S:FaMYB10 (Fragaria × ananassa) had significant increases of anthocyanins in fruits and leaves. HPLC analysis of fruits and leaves showed 70% increase of anthocyanin content in 35S:FaMYB10 transgenic lines compared with the wild-type controls. In transient transactivation assays, FaMYB10 and FvMYB10 (Fragaria vesca), co-expressed with AtbHLH2 (Arabidopsis thaliana), strongly activated the AtDFR promoter. By using the available Fragaria genome, transient expression of gene candidates, and stable transgenics, use of strawberry can provide rapid assessment of fruit gene function.
Kui Lin-Wang, , Yuhui Liu, , Espley, R.V., Karunairetnam, S., McGhie, T.K., Hellens, R.P. and Allan, A.C. (2014). REGULATION OF ANTHOCYANIN BIOSYNTHESIS IN STRAWBERRY (FRAGARIA SP.) BY OVER-EXPRESSION OF A KEY TRANSCRIPTION FACTOR. Acta Hortic. 1048, 137-142
Fragaria × ananassa, Fragaria vesca, anthocyanin, MYB transcription factor