CLONING AND EXPRESSION ANALYSIS OF TWO MADS-BOX GENES FROM STRAWBERRY FRUIT
A MADS-box protein gene named FaMADS1 was isolated from fruit tissue of strawberry (Fragaria ×ananassa Duch. Toyonoka) by RT-PCR and RACE. The result of the sequencing showed that the nucleotide sequence of FaMADS1 gene has 98% similarity with the Fv-MADS-9 gene sequences from the GenBank. The cDNA gene sequence is 1,167 bp in length with a 747 bp open reading frame encoding 249 amino acid proteins which displayed the structure of typical plant MADS-box proteins, and 100 bp in the 5UTR, 320 bp in the 3UTR, and 30 bp in the poly (A) tail. The SqRT-PCR experiment showed that the FaMADS1 gene is only expressed in fruit tissue, with no expression detected in flower organs. The expression levels in different stages of fruit development and ripening are from low in young flower receptacles to high as fruit receptacles begin turning red with brown achenes, and then expression is lower at the full red fruit stage. Another MADS-box protein gene named FaMADS2 from strawberry fruit was simultaneously cloned by RT-PCR and 3RACE. The cDNA sequence length is 870 bp with a 617 bp coding frame, 254 bp in the 3UTR, and 27 bp in the poly (A) tail. The nucleotide sequence of the FaMADS2 gene has 96% identity with the FcMADS2 gene sequence. The expression pattern of FaMADS1 and FaMADS2 genes is different in tissues and stages of berry development. FaMADS2 expression appears to be high in flower organs, and shows two peaks of expression in the young flower receptacle and white receptacle stages. This information provides new clues to aid in understanding the ripening process in strawberry fruit.
Zhou, H.C., Kang, Z.R., Zhao, X., Li, G. and Li, L. (2014). CLONING AND EXPRESSION ANALYSIS OF TWO MADS-BOX GENES FROM STRAWBERRY FRUIT. Acta Hortic. 1049, 349-353
strawberry (Fragaria ×ananassa Duch.), MADS-box gene, gene cloning, semi-quantitative RT-PCR