BIO-CONTROL BACILLUS STRAIN TS02 FOR CONTROL OF STRAWBERRY POWDERY MILDEW AND ITS MOLECULAR IDENTIFICATION

Chong Chen, Yu Wei, Tengfei Fan, Wen Zhang, Guozhen Zhang, Qizhi Liu, Haigeng Liu, Jieming Wang , Lusheng Zhang
Powdery mildew of strawberry is a major limitation for strawberry production in most strawberry growing areas of the world. Bio-control management is a good way to cope with this disease. Bacillus TS02 is a bio-control strain which was isolated from soil. The TS02 bio-controlling experiments were done according to the standard field experimental rule of pesticide controlling effect of China. The results indicate that TS02 can control strawberry powdery mildew. For the fermented liquid of TS02, above 3×107CFU/ml live bacteria concentration can achieve ideal effects, the best effect is 52.23% with 3×109CFU/ml live bacteria. There were 48.98, 34.89 and 24.63% bio-control effects respectively with the bacteria fermented liquid, pure live bacteria and filtrated bacteria fermented liquid, respectively, on strawberry powdery mildew, which showed the corporate results of live bacteria and secretion of bacteria. On the basis of the field experiments, the bio-control mechanism was studied in laboratory with the leaves in vitro. There was 100% growth inhibition by TS02 on Sphaerotheca macularis, TS02 can inhibit infection by the pathogen and control the strawberry disease.
TS02 was identified as a new strain of Bacillus cereus based on the homology of its 16SrRNA sequences to the reference strains registered in Genbank. TS02 formed a monophyletic group with strains of the B. cereus complex group in the 16SrDNA sequence analysis, and shared similarity values of 99.9% with the Bacillus cereus strains in the sequence analysis.
Chong Chen, , Yu Wei, , Tengfei Fan, , Wen Zhang, , Guozhen Zhang, , Qizhi Liu, , Haigeng Liu, , Jieming Wang , and Lusheng Zhang, (2014). BIO-CONTROL BACILLUS STRAIN TS02 FOR CONTROL OF STRAWBERRY POWDERY MILDEW AND ITS MOLECULAR IDENTIFICATION. Acta Hortic. 1049, 607-611
DOI: 10.17660/ActaHortic.2014.1049.94
https://doi.org/10.17660/ActaHortic.2014.1049.94
strawberry powdery mildew, bio-control, identification of 16S rDNA
English

Acta Horticulturae