CHARACTERIZATION OF ERWINIA AMYLOVORA STRAINS ISOLATED FROM QUINCE TREES IN SERBIA USING REP-PCR METHOD
Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper.
In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia.
First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots.
Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits (Williams). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose.
After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.
Gavrilović, V., Ivanović, Z., Popović , T. and Zivković, S. (2014). CHARACTERIZATION OF ERWINIA AMYLOVORA STRAINS ISOLATED FROM QUINCE TREES IN SERBIA USING REP-PCR METHOD. Acta Hortic. 1056, 169-172
DOI: 10.17660/ActaHortic.2014.1056.26
https://doi.org/10.17660/ActaHortic.2014.1056.26
DOI: 10.17660/ActaHortic.2014.1056.26
https://doi.org/10.17660/ActaHortic.2014.1056.26
quince, pathogenicity, biochemical characteristics, REP-PCR
English
1056_26
169-172
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Fruits