OPTIMIZATION OF LYOPHILIZATION TECHNOLOGY FOR PURIFICATION AND STABILIZATION OF ANTHOCYANINS FROM ELDERBERRY FRUITS

I. Salamon, D. Grulova, M. Hancianu, O. Cioanca
Natural medicinal compounds of plant origin have numerous perceived advantages in selected therapeutical applications compared with the synthetic chemistry. With regard to general isolation of these compounds (secondary metabolites of plants), distillation and extraction methods are most often used. The technique of lyophilization (freeze drying) has certain advantages over distillation or extraction technologies, especially with unstable compounds such as anthocyanins. The aim of this research was the optimization of a lyophilization process with acetone extracts of elderberry (Sambucus nigra) fruits. The device Lyophil SMART LYO 2 from the German producer GEA was used for the lyophilization. The work consisted of two parts: optimizing the dilution of samples by deionized water for mechanical bottle line filling (which also affected final product quality), and optimizing the lyophilization procedure to achieve the final product in the form of a dry powdered tablet at the end of the process. The optimal dilution for the elderberry extract was 4.7:1. The program for the lyophilization of elderberry extract was optimized in 11 steps, where temperature and pressure in time were changed. The basic research in its various stages confirmed the assumption that isolated natural substances (anthocyanins) can be extracted from plant material by freeze-drying, thus stabilizing their biological activities.
Salamon, I., Grulova, D., Hancianu, M. and Cioanca, O. (2015). OPTIMIZATION OF LYOPHILIZATION TECHNOLOGY FOR PURIFICATION AND STABILIZATION OF ANTHOCYANINS FROM ELDERBERRY FRUITS. Acta Hortic. 1061, 245-252
DOI: 10.17660/ActaHortic.2015.1061.27
https://doi.org/10.17660/ActaHortic.2015.1061.27
Sambucus, extract, freeze-drying, dilution, conditions
English

Acta Horticulturae