RAPID DIAGNOSTIC DETECTION OF PLUM POX VIRUS BY ISOTHERMAL AMPLIFYRP® AND BY IMMUNOSTRIP®

S. Zhang, M. Ravelonandro, M. Chambers, P. Briard, M. Masson, M. Amato, A. Vrient
Plum pox virus (PPV) is a member of the Potyviridae family and causes a very devastating plum pox or sharka disease in stone fruit species worldwide. Due to its wide spread and economic importance, PPV has become a highly regulated plant pathogen and there is a need for rapid and reliable diagnostic detection of PPV for the purpose of quarantine and control. Agdia Inc. has recently developed AmplifyRP®: and ImmunoStrip®: tests for PPV detection. The AmplifyRP®: tests are nucleic acid-based isothermal detections of viral RNA using a recombinase polymerase amplification technology. In contrast, PPV ImmunoStrip®: is a protein-based immunological detection of viral protein. Each of these tests can detect all nine PPV strains currently known (PPV-An, PPV-C, PPV-CR, PPV-D, PPV-EA, PPV-M, PPV-Rec, PPV-T or PPV-W) directly from crude woody plant extracts. The AmplifyRP®: and ImmunoStrip®: tests were evaluated and compared to conventional ELISA. The results demonstrated that these new tests of AmplifyRP®: and ImmunoStrip®: are rapid and simple. The whole process from sample preparation to result can be completed in as little as 30 min. All these three tests do not need thermal cycling and expensive equipment and can be performed both in laboratories and in field.
Zhang, S., Ravelonandro, M., Chambers, M., Briard, P., Masson, M., Amato, M. and Vrient, A. (2015). RAPID DIAGNOSTIC DETECTION OF PLUM POX VIRUS BY ISOTHERMAL AMPLIFYRP® AND BY IMMUNOSTRIP®. Acta Hortic. 1063, 167-172
DOI: 10.17660/ActaHortic.2015.1063.23
https://doi.org/10.17660/ActaHortic.2015.1063.23
isothermal amplification, lateral flow assay, Plum pox virus, real-time assay, recombinase polymerase amplification
English

Acta Horticulturae