MICRO-SHOOT TIP GRAFTING AS TOOL FOR PRODUCTION OF DISEASE FREE FOUNDATION BLOCK OF CITRUS RETICULATE BLANCO 'KHASI' MANDARIN

Rakesh Sanabam, Pratap J. Handique, Sunitibala Huidrom
This study was carried out to produce viral disease free scion bank of ‘Khasi’ mandarin (Citrus reticulate Blanco) using four Citrus species namely ‘Kachai’ lime (Citrus jambhiri Lush), Champra maounthabi (Citrus jambhiri Lush), Solom (Citrus sps.) and Phouheiree (Citrus sps) through micrografting techniques as viral disease affects the longevity of citrus orchards and quality of fruits. Success of micrografting depended on the type of cut for scion insertion, age of seedlings used as rootstock, conditions of light or darkness, concentration of sucrose used and also on rootstock types used for micrografting. Frequency of success improved considerably from 33.62% at 3% sucrose to 51.62% at 6% sucrose with 0.5 mg/L benzyl amino purine (BAP) in Murashige and Tucker (MT) liquid media using 0.5 mm scion size. Response of micrografting was significantly improved when inverted-T type cut was used for scion insertion giving 37.59% compared to 28.38% success in that of wedge type of cut. The highest rate of success was obtained when scions were grafted onto ‘Champra maounthabi’ with success rate of 40.74% followed by ‘Phouheiree’, ‘Solom’ and ‘Kachai’ lime, with rates of 34.26, 32.24 and 27.53%, respectively. Analysis of 8-10 months old micrografted plants for the presence of the graft transmissible Citrus tristeza virus (CTV) following double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) was performed, giving negative results for the virus.
Rakesh Sanabam, , Pratap J. Handique, and Sunitibala Huidrom, (2015). MICRO-SHOOT TIP GRAFTING AS TOOL FOR PRODUCTION OF DISEASE FREE FOUNDATION BLOCK OF CITRUS RETICULATE BLANCO 'KHASI' MANDARIN. Acta Hortic. 1065, 643-647
DOI: 10.17660/ActaHortic.2015.1065.80
https://doi.org/10.17660/ActaHortic.2015.1065.80
nucellar, virus, scion, rootstock, Murashige and Tucker (MT) and graft
English

Acta Horticulturae