MONITORING CITRUS TRISTEZA VIRUS (CTV) IN ALGERIA AND CHARACTERIZATION OF LOCAL ISOLATES

Djamila Larbi, Hadjira Belkahla, Khaled Djelouah, Anna M. D'Onghia
During the period 2009-2012, the main Algerian citrus growing areas located in the Mitidja region were surveyed using a hierarchical sampling protocol developed in the Mediterranean Agronomic Institute of Bari (MAIB-HS) to assess the presence of Citrus tristeza virus (CTV). Flowers and leaves from the selected trees were analyzed by Direct Tissue Blot Immunoassay (DTBIA). About 25.6% of the 2193 trees tested were CTV-infected, with highest CTV infection rate being found in ‘Common’ mandarin trees (72.1%), followed by Jaffa orange (58.8%) and to a lesser extent by lemon and common clementine. Clear tristeza decline symptoms were observed during the survey in some trees propagated on the sour orange rootstock. A few CTV-infected samples selected for indexing on the universal indicator Mexican lime, induced in this host vein clearing symptoms. Single-strand conformation polymorphism (SSCP) and sequence analysis of the coat protein gene of 39 selected Algerian CTV isolates showed 99% similarity with the Spanish mild CTV isolate T385.
Djamila Larbi, , Hadjira Belkahla, , Khaled Djelouah, and Anna M. D'Onghia, (2015). MONITORING CITRUS TRISTEZA VIRUS (CTV) IN ALGERIA AND CHARACTERIZATION OF LOCAL ISOLATES . Acta Hortic. 1065, 767-771
DOI: 10.17660/ActaHortic.2015.1065.96
https://doi.org/10.17660/ActaHortic.2015.1065.96
Algeria, CTV survey, DTBIA, PCR, SSCP
English

Acta Horticulturae