EFFECT OF CARBON SOURCE ON PROTOCORM-LIKE BODY INDUCTION, PROLIFERATION AND REGENERATION IN DENDROBIUM SNOWFLAKE 'RED STAR'

W. Udomdee, P.J. Wen, S.W. Chin, F.C. Chen
An efficient micropropagation procedure is established for a nobile Dendrobium hybrid, D. Snowflake ‘Red Star’, by induction of protocorm-like bodies (PLBs). PLBs could be induced from shoot tips of axillary buds on Hyponex-based medium and the greatest induction rate occurred on medium supplemented with 0.03 M sucrose. Among six types of carbon source tested, sucrose, glucose, fructose and maltose were better than sorbitol and mannitol for PLBs proliferation. For PLB proliferation, in terms of fresh weight increase, sucrose at 0.06-0.09 M and maltose at 0.06-0.12 M were better than other concentrations. For conversion of PLBs into shoots, maltose, sucrose, sorbitol, glucose and fructose showed number and fresh weight of regenerated shoot better than mannitol. In addition, the number of regenerated shoot was better on medium supplemented with maltose at 0.03-0.12 M.
Udomdee, W., Wen, P.J., Chin, S.W. and Chen, F.C. (2015). EFFECT OF CARBON SOURCE ON PROTOCORM-LIKE BODY INDUCTION, PROLIFERATION AND REGENERATION IN DENDROBIUM SNOWFLAKE 'RED STAR'. Acta Hortic. 1078, 113-120
DOI: 10.17660/ActaHortic.2015.1078.15
https://doi.org/10.17660/ActaHortic.2015.1078.15
nobile Dendrobium, PLB, sucrose, carbon source
English

Acta Horticulturae