THE PRODUCTION OF TRANSGENIC CARROT PLANTS RESISTANT TO HERBICIDE BASTA
A construct containing two chimeric genes, Cauliflower mosaic virus 35s - (bar) and nopaline synthase -neomycin phosphotransferase (nptII), was introduced into carrot cultivar Nantskaya 4 using Agrobacterium tumefaciens-mediated transformation procedure. A number of transgenic carrot plants showing the stable integration of the bar gene were produced from embryogenic callus culture. Most of the obtained transgenic plants showed complete herbicide resistance at 0.05-0.50% Basta. Thus, introduction of the bar gene into a carrot cultivar can allow efficient production of herbicide-resistant transgenic carrot with commercial potential.
Sidorova, T., Miroshnichenko, D., Dolgov, S. and Tjukavin, G. (2015). THE PRODUCTION OF TRANSGENIC CARROT PLANTS RESISTANT TO HERBICIDE BASTA. Acta Hortic. 1083, 133-137
Agrobacterium, bar gene, herbicide resistance, phosphinothricin acetyl transferase